Reporter

Part:BBa_K911009:Design

Designed by: Fai Tsang   Group: iGEM12_Cambridge   (2012-09-24)


Fluorescent ratiometric construct for standardizing promoter output


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick is best used with a shuttle vector that can function in both E. coli and B. subtilis, which should also have preferably different antibiotic markers in E. coli and B. subtilis.


Source

This biobrick is composed entirely of existing biobricks: GsiB (K143020), eCFP (E0020), terminator (B0015), Pveg + SpoVG (K143053), eYFP (E0030) and a second terminator (B0010). It was constructed first on the E. coli- B. subitilis shuttle vector pJS130, then moved onto pSB1C3 for submission.

References